Plasmid_Backbone

Part:BBa_K4516007:Design

Designed by: Kong Yangyang   Group: iGEM22_Jiangsu_United   (2022-07-19)


pGL3pro-backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 2966
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 10
    Illegal BamHI site found at 320
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 58
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 444
    Illegal NgoMIV site found at 2513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1536
    Illegal SapI site found at 453


Design Notes

No


Source

No

References